Wei Zhuo¹ , Yan Liu¹ , Juan Bai² , Rong Xiang³ , Shenge Lu¹ , Fengming Ren¹

1 Chongqing Institute of Medicinal Plant Cultivation, Chongqing, 408435, china;
2 Economic Crops Technology Extension Station of Chongqing Yubei District, Chongqing, 401120, China;
3 Yunyang Saifeng Agricultural Development Co., Ltd., Chongqing, 404500, China
Author    Correspondence author
International Journal of Molecular Ecology and Conservation, 2020, Vol. 10, No. 1   
Received: 19 Jun., 2020    Accepted: 22 Jun., 2020    Published: 10 Jul., 2020

This article was first published in 《分子植物育种》(ISSN1672-416X，CN46-1068/S) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

In order to evaluate the identification ability of nrDNA ITS sequences on Torreya , this study used ITS sequence and four different analysis methods(BLAST, K2P genetic distance, SNP analysis, NJ tree) to identify species of Torreya, and established phylogenetic trees to discuss their phylogenetic relationships. The results showed that the length of 48 ITS sequences was 1 095 bp~1 105 bp, the average intraspecies genetic distance was 0.001 6 and the average interspecies genetic distance was 0.015 0. At the species level, BLAST alignment and NJ tree had the highest efficiency in Torreya. The SNP loci analysis could effectively identify T. grandis cv. ‘Merrillii’ and T. grandis. The analysis of genetic relationship showed that all the other species of Torreya were single-line branches, but the quince tree of T. yunnanensis and T. fargesii were clustered into a cluster in the ML tree, indicating that the two were closely related. This provided evidence for the nuclear genome sequence proposed the incorporation of T. yunnanensis and T. fargesii. This study showed that ITS sequence could be used as a DNA barcode for the identification of Torreya, and providing reference for the identification and systematic relationship of Torreya.

DNA barcode; Torreya; ITS sequence; Identification