Chunxiang Ma

Modern Agricultural Research Center, Cuixi Academy of Biotechnology, Zhuji, 311800, China
Author    Correspondence author
GMO Biosafety Research, 2024, Vol. 15, No. 4   
Received: 05 Jul., 2024    Accepted: 14 Aug., 2023    Published: 26 Aug., 2024

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

This study analyzed the establishment and optimization of wheat tissue culture system, and analyzed the effects of key technical parameters such as explant type, genotype adaptability, hormone ratio on regeneration efficiency and genetic stability; described the latest application progress of gene gun method, Agrobacterium-mediated method and gene editing technology (such as CRISPR/Cas9) in wheat gene transformation and trait improvement, especially the research results in disease resistance, stress resistance, nutritional quality, etc. In addition, this study further explored the phenotypic evaluation, genetic stability, ecological adaptability and safety assessment requirements of wheat biotechnology improved materials in multi-environment testing from greenhouse to field. The study found that wheat biotechnology is gradually realizing industrial transformation from laboratory to field, but it still needs to solve problems such as low tissue culture efficiency, poor genotype adaptability, public acceptance and regulatory policy challenges. An efficient and accurate multidisciplinary integrated breeding platform should be established to achieve the goals of modern precision breeding of wheat and sustainable agricultural development. This study provides a scientific theoretical basis and practical guidance for promoting precision breeding of wheat, which will help accelerate the cultivation and commercial promotion of new varieties of stress-resistant, high-yield and high-quality wheat.

Wheat; Tissue culture; Gene transformation; Gene editing; Field evaluation