Meng Wang , Dan Luo , Limei Yi , Zongchao Liao , Yanning Su , Jiaqi Xia , Xinquan Zhang , Gang Nie

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China
Author    Correspondence author
International Journal of Molecular Evolution and Biodiversity, 2022, Vol. 12, No. 4   doi: 10.5376/ijmeb.2022.12.0004
Received: 25 Apr., 2022    Accepted: 02 May, 2022    Published: 10 May, 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Wang M., Luo D., Yi L.M., Liao Z.C., Su Y.N., Xia J.Q., Zhang X.Q., and Nie G., 2022, Genetic diversity analysis of Roegneria kamoji population from Sichuan and Chongqing based on ISAP molecular markers, International Journal of Molecular Evolution and Biodiversity, 12(4): 1-10 (doi: 10.5376/ijmeb.2022.12.0004)

There are abundant forage resources in southwest China, among which the wild Roegneria kamoji Ohwi are widely distributed, especially in Sichuan and Chongqing province. Aim to figure out the genetic structure of wild R. kamoji Ohwi in these places, providing the theoretical basis for germplasm resources collection, utilization and breeding, also exploring the genetic diversity of wild R. kamoji Ohwi. In this experiment, the genetic diversity of 80 wild R. kamoji Ohwi from Sichuan and Chongqing were analyzed by the new molecular maker ISAP. Results showed that a total of 102 distinct and recognizable bands were amplified from 20 pairs of primers, with 97 polymorphic bands accounting for 93.14%. The PIC average value of each ISAP site was 0.255. The results of cluster analysis showed that the germplasm resources of 80 wild R. kamoji Ohwi were divided into 3 groups when GS was 0.815, and the clustering among the materials showed a certain correlation with geographical sources. This study might contribute to provide a theoretical basis and materials for the genetic breeding of wheat family crops in the future.

Roegneria kamoji Ohwi; ISAP; Molecular marker; Genetic diversity