Research Report

Analysis of Genetic Diversity and Relationship of Jacaranda mimosifolias Based on ISSR Molecular Markers Technique  

Xuefeng Liu1 , Guo Liu1 , XiaoMei Li2 , Peijian Zhang1 , Min Huang1 , Liqiong Gao1 , Liang Fang1 , Guowu Zhang1
1 China Eucalypt Research Centre, Zhanjiang, 524022, China
2 Guangdong Ocean University Cunjin College, Zhanjiang, 524094, China
Author    Correspondence author
International Journal of Molecular Evolution and Biodiversity, 2022, Vol. 12, No. 1   doi: 10.5376/ijmeb.2022.12.0001
Received: 10 Feb., 2022    Accepted: 17 Feb., 2022    Published: 24 Feb., 2022
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This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Liu X.F., Liu G., Li X.M., Zhang P.J., Huang M., Gao L.Q., Fang L., and Zhang G.W., 2022, Analysis of genetic diversity and relationship of Jacaranda mimosifolias based on ISSR molecular markers technique, International Journal of Molecular Evolution and Biodiversity, 12(1): 1-8 (doi: 10.5376/ijmeb.2022.12.0001)


The genetic diversity and relationship of Jacaranda mimosifolias from 19 provenances including 21 germplasms in China were studied by ISSR molecular markers technique. 53 bands were amplified by using 10 selected primers from all the 33 primers, of which 44 bands were polymorphic, with the polymorphism ratio of 83.02%. Each primer could amplify 5.3 bands in average. The average number of tested alleles (Na), effective alleles (Ne), Nei’s gene diversity index (H) and Shannon’s information index (I) were 1.83, 1.45, 0.27, 0.40, respectively. ISSR molecular marker cluster analysis research shows 21 J. mimosifolias germplasm resources could be divided into two groups, and the genetic diversity is high. Their genetic relationship is not strongly related to geographical origin of cultivation area. In this study, the relatively accurately molecular identification of the germplasm resources of J. mimosifolias was carried out, which will provide a theoretical basis for the breeding of the improved varieties.

Jacaranda mimosifolia; ISSR; Genetic diversity; Genetic relationship
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International Journal of Molecular Evolution and Biodiversity
• Volume 12
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