Research Report
Analysis of Genetic Diversity and Relationship of Jacaranda mimosifolias Based on ISSR Molecular Markers Technique
2 Guangdong Ocean University Cunjin College, Zhanjiang, 524094, China
Author Correspondence author
International Journal of Molecular Evolution and Biodiversity, 2022, Vol. 12, No. 1 doi: 10.5376/ijmeb.2022.12.0001
Received: 10 Feb., 2022 Accepted: 17 Feb., 2022 Published: 24 Feb., 2022
Liu X.F., Liu G., Li X.M., Zhang P.J., Huang M., Gao L.Q., Fang L., and Zhang G.W., 2022, Analysis of genetic diversity and relationship of Jacaranda mimosifolias based on ISSR molecular markers technique, International Journal of Molecular Evolution and Biodiversity, 12(1): 1-8 (doi: 10.5376/ijmeb.2022.12.0001)
The genetic diversity and relationship of Jacaranda mimosifolias from 19 provenances including 21 germplasms in China were studied by ISSR molecular markers technique. 53 bands were amplified by using 10 selected primers from all the 33 primers, of which 44 bands were polymorphic, with the polymorphism ratio of 83.02%. Each primer could amplify 5.3 bands in average. The average number of tested alleles (Na), effective alleles (Ne), Nei’s gene diversity index (H) and Shannon’s information index (I) were 1.83, 1.45, 0.27, 0.40, respectively. ISSR molecular marker cluster analysis research shows 21 J. mimosifolias germplasm resources could be divided into two groups, and the genetic diversity is high. Their genetic relationship is not strongly related to geographical origin of cultivation area. In this study, the relatively accurately molecular identification of the germplasm resources of J. mimosifolias was carried out, which will provide a theoretical basis for the breeding of the improved varieties.
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