Research Article

Genetic Diversity of Cultivated A. auricula by Comprehensive Analysis of rDNA-ITS and SRAP  

Kunang Liu , Hong Ma , Meng Liu , Genwei Zhang , Shuli Yin , Shusheng Li
Institute of Biology, Hebei Academy of Sciences, Shijiazhuang, 050081, China
Author    Correspondence author
International Journal of Molecular Evolution and Biodiversity, 2022, Vol. 12, No. 2   doi: 10.5376/ijmeb.2022.12.0002
Received: 14 Apr., 2022    Accepted: 27 Apr., 2022    Published: 08 May, 2022
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This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Liu K.A., Ma H., Liu M., Zhang G.W., Yin S.L., and Li S.S., 2022, Genetic diversity of cultivated A. auriculala by comprehensive analysis of rDNA-ITS and SRAP, International Journal of Molecular Evolution and Biodiversity, 12(2): 1-10 (doi: 10.5376/ijmeb.2022.12.0002)


The genetic diversity of 15 A. auricula cultivars in North China was analyzed by strain affinity test, rDNA its and SRAP cluster analysis. The genetic relationship among the strains was clarified, which provided theoretical basis for the cultivation, genetic breeding and germplasm resources protection of A. auricula. The results showed that most of the antagonistic reactions of 15 A. auricula strains showed two kinds of incompatibility, i.e. non uplift type and ravine type, among which 5 pairs had no antagonistic reaction between A. auricula varieties, while ‘Jiyou No.1’ had obvious antagonistic reaction with other strains; rDNA its cluster analysis divided 15 A. auricula strains into three categories, the genetic distance between including ‘Heimuer-1’ and ‘Heimuer-2’, ‘Heiweibanjin’ and ‘Longjianghei+’, ‘Diaodai’ and ‘Heishan’ were 0, while that between ‘Jiyou No.1’ and other varieties was larger. The 9 SRAP primers amplified bands were rich in polymorphism, and the polymorphic fragments accounted for 79.8% of the total. The 15 cultivated species of A. auricula were divided into 3 categories by cluster analysis. The similarity coefficient among the strains of ‘Heiweibanjin’ and ‘Longjianghei+’ was 95%, which indicated that there was no genetic difference between them. The genetic relationship between ‘Jiyou No.1’ and other strains was far.

A. auricula; rDNA-ITS; SRAP; Genetic diversity
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International Journal of Molecular Evolution and Biodiversity
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